Coursera cryo em software

Tomography data collection and reconstruction coursera. Here the basic concepts for techniques such as electron microscopy em, cryo tem, flow cytometry, atomicforce microscopy and nanoparticle tracking analysis will be covered and some guidelines will be presented. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic. This class covers the fundamental principles underlying cryo electron microscopy cryo em starting with the basic anatomy of electron microscopes, an introduction to fourier transforms, and the principles of image formation. This module highlights some of the different techniques used to characterize extracellular vesicles. And im going to draw as more nearly like the original tree that we were trying to image in the first place.

Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo em. So this is the process of ctf correction, and it can work very well. The class is perfect as a primer for anyone who is about to be trained as a cryo electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo em meetings. At class central, we get that question so often that i wrote a guide to answer it. One of the most important things we do in my lab is cryo electron microscopy and 3d electron microscopy. The rapidly growing interest in cryoelectron microscopy cryoem, driven by major technological advances in recent years, has increased the demand for.

The software solves a set of equations, where the knowns are the. Lectures by grant jensen, professor of biology, caltech. Cryo em allows biological samples such as macro molecular complexes, viruses or even whole cells to be imaged in a near native frozen hydrated state. This class covers the fundamental principles underlying cryoelectron microscopy cryoem starting with the basic anatomy of. And then finally the last step of ctf correction is to calculate the fourier transform of this ctf corrected version and produce what well call a ctf corrected image. Generally speaking, coursera courses are free to audit but if you want to access. Here are nearly 1,200 coursera courses that are still.